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Hematoxylin and Eosin Infographic

Shows the mechanism of action of hematoxylin and eosin staining

Jerry Clarin, HT (ASCP)

• 1 min read
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Infographic showing molecular mechanism of H&E staining: hematoxylin-aluminum binding to DNA phosphates, eosin binding electrostatically to proteins.
How H&E Staining Actually Works
Infographic showing hematoxylin staining mechanism in four steps: oxidation to hematein, aluminum chelation, bluing reaction, and DNA phosphate binding.
Four Steps Behind the Blue
Infographic of eosin staining mechanism in five panels: anionic dye structure, exposed protein residues, ionic bonding, pH dependence, hydrophobic forces.
Why Eosin Stains Pink

Thin Sections is written by Jerry Clarin, HT (ASCP). The views expressed are his own and do not represent any employer.